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McClelland Protocols

 Faculty>Michael McClelland>McClelland Lab
 


Protocols

Array-based analysis of cistrons under selection (ABACUS)

DNA isolation (Sigma GenElute)

RNA isolation (Promega SV Total)

DNA labeling (Klenow and N6)

RNA labeling (Superscript and N6)

Hybridization (with formamide)

ChIP/chip

 

Arrays

Salmonella 17k ORF array info 

Salmonella 17k ORF array design STv7E (8Mb .xls spreadsheet)

Salmonella 12k Oligo array design SPOC3 (5Mb .xls spreadsheet)

Salmonella 384k Oligo array design MMCCSAL05 (49Mb .txt file)

 

Resources

List of MMCC's specific knockout mutants from S. enterica sv Typhimurium 14028 (2Mb .xls spreadsheet)

MMCC S. enterica sv Typhimurium 14028 single-gene Wanner mutant plate collection (500kb .xls spreadsheet)

Typhimurium LT2-specific oligos designed by MMCC lab (13Mb .xls spreadsheet)

 


 

 

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